INSA-Lyon/26 July 2010

From 2010.igem.org





July 26


Agarose gel electrophoresis to control the digestion of 1F and 24A realized on 07/23: Band 1F --> ok, Band 24A --> weak intensity

Extraction and purification of DNA contained on the band 24A with a kit (concentrated in 20uL)


Ligations :

  1. 18A/ 1N
  2. 14K/ 23L
  3. 1F/24A: ratio 2:1 --> 10/5µL

Digestions :

  1. L1 by S+P and E+P
  2. L2 by X+P

Agarose gel electrophoresis --> no result

Pre culture of 18A/1N








June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31