INSA-Lyon/26 July 2010
From 2010.igem.org
July 26
Agarose gel electrophoresis to control the digestion of 1F and 24A realized on 07/23: Band 1F --> ok, Band 24A --> weak intensity
Extraction and purification of DNA contained on the band 24A with a kit (concentrated in 20uL)
Ligations :
- 18A/ 1N
- 14K/ 23L
- 1F/24A: ratio 2:1 --> 10/5µL
Digestions :
- L1 by S+P and E+P
- L2 by X+P
Agarose gel electrophoresis --> no result
Pre culture of 18A/1N
|
|
|
|
|