INSA-Lyon/24 August 2010
From 2010.igem.org
August 24
The ligation 1N/L2 in backbone Cl didn't succeed
Extraction and Digestion :
- ligation 10L/4O
- Ligation 1F/24A (2 colonies)
- Ligation 8C/14K
- 8C
- 14K
Gel electrophoresis --> the gel was not readable
Digestion and Ligation with the ozyme kit (4µL of each DNA) :
- 1N+L2 in Cm backbones
- 40/10L in Tet backbones
- I0500/24A in Tet backbones
- 8C/14K in Tet backbones
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