INSA-Lyon/22 September 2010
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Revision as of 11:09, 1 October 2010
September 22nd
DNA extraction of the clone of the ligation from the 21/09 of phaA/phaB
Agarose gel electrophoris for weight verification :
- 1F/24A : the clone (mise en )collection is OK
- Curli/22B : the clone (mise en )collection is OK
- 8C/14K : problem with the extraction (no trace of DNA on the gel)
Digestion and purification on agarose gel:
- 8C, 1F/24A : with S+P
- 14K with X+P
Ligation overnight of 8C/14K and 1F/24A/14K
Liquid cultur of 18A/24A, 1F/24A, 14K, I0500 and PHL818
Measure of fluorescence and DO of the liquid cultur of PHL1273 of the 20/09. Start of 6 new culturs :
- 3 in a water bath at 35°C, shaking speed 100 rpm
- 3 in a water bath at 36°C, shaking speed 100 rpm
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