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| <!-- {{INSA-Lyon_style}} --> | | <!-- {{INSA-Lyon_style}} --> |
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| {{INSA-Lyon/stylegroningen}} | | {{INSA-Lyon/stylegroningen}} |
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| {{INSA-Lyon/header}} | | {{INSA-Lyon/header}} |
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| {{INSA-Lyon/maincontent}} | | {{INSA-Lyon/maincontent}} |
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| {{INSA-Lyon/menugauche}} | | {{INSA-Lyon/menugauche}} |
| + | {{INSA-Lyon/menunotebook}} |
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| <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en"> | | <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en"> |
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| </script> | | </script> |
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- | <div class="colonnegauche">
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- | <div id="menug">
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- | <ul>
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- | <li><a href="/Team:INSA-Lyon/Project/Theory" class="cteal"> > Theory</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Strategy" class="slateb"> > Strategy</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Results" class="yellow"> > Results</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li>
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- | </div>
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- | <li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modelling</a></li>
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- | <li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li>
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| <p>DNA extraction of the clone of the ligation from the 21/09 of phaA/phaB </p><br> | | <p>DNA extraction of the clone of the ligation from the 21/09 of phaA/phaB </p><br> |
- | <p>Agarose gel electrophoris for weight verification : <br> | + | <p>Agarose gel electrophoresis for weight verification : <br> |
| <ol id="list_extra"> | | <ol id="list_extra"> |
| <li>1F/24A : the clone (mise en )collection is OK</li> | | <li>1F/24A : the clone (mise en )collection is OK</li> |
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| </ol><br> | | </ol><br> |
| </p> | | </p> |
- | <p>Ligation overnight of 8C/14K and 1F/24A/14K</p><br> | + | <p>Ligation overnight of : <br> |
- | <p>Liquid cultur of 18A/24A, 1F/24A, 14K, I0500 and PHL818</p><br> | + | <ol id="list_extra"> |
- | <p>Measure of fluorescence and DO of the liquid cultur of PHL1273 of the 20/09. Start of 6 new culturs : <br> | + | <li>8C/14K : one with only 14K from gel purification and one with the 8C and 14K from a gel purification</li> |
| + | <li>1F/24A/14K : one ligation</li> |
| + | </ol></p><br> |
| + | <p>Measure of fluorescence and DO of the liquid culture of PHL1273 of the 20/09. Start of 6 new cultures : <br> |
| <ol id="list_extra"> | | <ol id="list_extra"> |
| <li> 3 in a water bath at 35°C, shaking speed 100 rpm</li> | | <li> 3 in a water bath at 35°C, shaking speed 100 rpm</li> |
| <li> 3 in a water bath at 36°C, shaking speed 100 rpm</li> | | <li> 3 in a water bath at 36°C, shaking speed 100 rpm</li> |
- | </ol><br></p> | + | </ol><br></p><br> |
| + | <p>Gel purification on vial 3 of ompR234 PCR (DNA dilution 1/10): 20µl are deposited in a lane. 100mg agarose (in 200µl NT) are extracted with NucleoSpin ExtractII kit (Macherey-Nagel). Elution was done in 50µl, and sample was stored at -20°C.</p><br> |
| + | <p>5 liquid cultures in LB medium (5ml) supplemented with corresponding antibiotic: 14K (Amp), I0500 (Kan), 1F/24A (Tet) and 18A/24A (Cm), and PHL818 (Tet), 37°C overnight.</p> |
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September 22nd
DNA extraction of the clone of the ligation from the 21/09 of phaA/phaB
Agarose gel electrophoresis for weight verification :
Digestion and purification on agarose gel:
Ligation overnight of :
Measure of fluorescence and DO of the liquid culture of PHL1273 of the 20/09. Start of 6 new cultures :
Gel purification on vial 3 of ompR234 PCR (DNA dilution 1/10): 20µl are deposited in a lane. 100mg agarose (in 200µl NT) are extracted with NucleoSpin ExtractII kit (Macherey-Nagel). Elution was done in 50µl, and sample was stored at -20°C.
5 liquid cultures in LB medium (5ml) supplemented with corresponding antibiotic: 14K (Amp), I0500 (Kan), 1F/24A (Tet) and 18A/24A (Cm), and PHL818 (Tet), 37°C overnight.
June
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_June_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_June_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_June_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_June_2010&action=edit 6]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_June_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_June_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_June_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_June_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_June_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_June_2010&action=edit 13]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_June_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_June_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_June_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_June_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_June_2010&action=edit 20]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_June_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_June_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_June_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_June_2010&action=edit 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_June_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_June_2010&action=edit 26]
| [http://2010.igem.org/INSA-Lyon/27_June_2010 27]
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[http://2010.igem.org/INSA-Lyon/28_June_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_June_2010 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_June_2010&action=edit 30]
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July
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_July_2010&action=edit 4]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_July_2010&action=edit 5]
| [http://2010.igem.org/INSA-Lyon/6_July_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_July_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_July_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_July_2010 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_July_2010&action=edit 10]
| [http://2010.igem.org/INSA-Lyon/11_July_2010 11]
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[http://2010.igem.org/INSA-Lyon/12_July_2010 12]
| [http://2010.igem.org/INSA-Lyon/13_July_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_July_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_July_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_July_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_July_2010 17]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_July_2010&action=edit 18]
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[http://2010.igem.org/INSA-Lyon/19_July_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_July_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_July_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_July_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_July_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_July_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_July_2010 25]
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[http://2010.igem.org/INSA-Lyon/26_July_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_July_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_July_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_July_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_July_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_July_2010 31]
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August
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| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/1_August_2010&action=edit 1]
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[http://2010.igem.org/INSA-Lyon/2_August_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_August_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_August_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_August_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_August_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/8_August_2010&action=edit 8]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/9_August_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/10_August_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_August_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_August_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_August_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/14_August_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_August_2010&action=edit 15]
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[http://2010.igem.org/INSA-Lyon/16_August_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_August_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_August_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_August_2010 19]
| [http://2010.igem.org/INSA-Lyon/20_August_2010 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_August_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_August_2010&action=edit 22]
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[http://2010.igem.org/INSA-Lyon/23_August_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_August_2010 24]
| [http://2010.igem.org/INSA-Lyon/25_August_2010 25]
| [http://2010.igem.org/INSA-Lyon/26_August_2010 26]
| [http://2010.igem.org/INSA-Lyon/27_August_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_August_2010 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_August_2010&action=edit 29]
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[http://2010.igem.org/INSA-Lyon/30_August_2010 30]
| [http://2010.igem.org/INSA-Lyon/31_August_2010 31]
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September
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| [http://2010.igem.org/INSA-Lyon/1_September_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_September_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_September_2010 3]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/4_September_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_September_2010&action=edit 5]
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[http://2010.igem.org/INSA-Lyon/6_September_2010 6]
| [http://2010.igem.org/INSA-Lyon/7_September_2010 7]
| [http://2010.igem.org/INSA-Lyon/8_September_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_September_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_September_2010&action=edit 12]
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[http://2010.igem.org/INSA-Lyon/13_September_2010 13]
| [http://2010.igem.org/INSA-Lyon/14_September_2010 14]
| [http://2010.igem.org/INSA-Lyon/15_September_2010 15]
| [http://2010.igem.org/INSA-Lyon/16_September_2010 16]
| [http://2010.igem.org/INSA-Lyon/17_September_2010 17]
| [http://2010.igem.org/INSA-Lyon/18_September_2010 18]
| [http://2010.igem.org/INSA-Lyon/19_September_2010 19]
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[http://2010.igem.org/INSA-Lyon/20_September_2010 20]
| [http://2010.igem.org/INSA-Lyon/21_September_2010 21]
| [http://2010.igem.org/INSA-Lyon/22_September_2010 22]
| [http://2010.igem.org/INSA-Lyon/23_September_2010 23]
| [http://2010.igem.org/INSA-Lyon/24_September_2010 24]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_September_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_September_2010&action=edit 26]
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[http://2010.igem.org/INSA-Lyon/27_September_2010 27]
| [http://2010.igem.org/INSA-Lyon/28_September_2010 28]
| [http://2010.igem.org/INSA-Lyon/29_September_2010 29]
| [http://2010.igem.org/INSA-Lyon/30_September_2010 30]
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October
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| [http://2010.igem.org/INSA-Lyon/1_October_2010 1]
| [http://2010.igem.org/INSA-Lyon/2_October_2010 2]
| [http://2010.igem.org/INSA-Lyon/3_October_2010 3]
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[http://2010.igem.org/INSA-Lyon/4_October_2010 4]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/5_October_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/6_October_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/7_October_2010&action=edit 7]
| [http://2010.igem.org/INSA-Lyon/8_October_2010 8]
| [http://2010.igem.org/INSA-Lyon/9_October_2010 9]
| [http://2010.igem.org/INSA-Lyon/10_October_2010 10]
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[http://2010.igem.org/INSA-Lyon/11_October_2010 11]
| [http://2010.igem.org/INSA-Lyon/12_October_2010 12]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/13_October_2010&action=edit 13]
| [http://2010.igem.org/INSA-Lyon/14_October_2010 14]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/15_October_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/16_October_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/17_October_2010&action=edit 17]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/18_October_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/19_October_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/20_October_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/21_October_2010&action=edit 21]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/22_October_2010&action=edit 22]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/23_October_2010&action=edit 23]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/24_October_2010&action=edit 24]
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[http://2010.igem.org/wiki/index.php?title=INSA-Lyon/25_October_2010&action=edit 25]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/26_October_2010&action=edit 26]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/27_October_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/28_October_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/30_October_2010&action=edit 30]
| [http://2010.igem.org/wiki/index.php?title=INSA-Lyon/31_October_2010&action=edit 31]
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