INSA-Lyon/19 September 2010

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September 19th


Digestion (Ozymes enzymes) :

  1. Amp backbone : with E+P
  2. 14/12/13 : with E+P
  3. 14 : with X
  4. phaA(PCR) : with E+S
  5. phaB(PCR : with X+P

Liquid cultur of one clone of phasin-phasin-intein which appear on the ligation from the 17/09


Ligation of phaA/phaB


Agarose gel electrophoris for verification :(result)


DNA extraction and digestion whith E+P of Phasin-phasin_intein.Agarose gel electrophoris for verification : the ligation is not OK








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