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INSA-Lyon/19 September 2010
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Latest revision as of 20:00, 25 October 2010
September 19th
Digestion (Ozymes enzymes) :
- Amp backbone : with E+P
- 14/12/13 : with E+P
- 14 : with X
- phaA(PCR) : with E+S
- phaB(PCR : with X+P
Liquid cultur of one clone of phasin-phasin-intein which appear on the ligation from the 17/09
Ligation of phaA/phaB
Agarose gel electrophoris for verification :(result)
DNA extraction and digestion whith E+P of Phasin-phasin_intein.Agarose gel electrophoris for verification : the ligation is not OK
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