INSA-Lyon/18 September 2010

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September 18th


DNA extraction :

  1. I0500 : 2 clones (plates from the 17/08 and 13/08)
  2. 1F/24A : 3 clones
  3. curli/22B :2 clones
  4. 4O/10L : 1 clone


PHL818 liquid culture in LB-Tet (GC) 8:45 AM - 11:15 AM: reaching a DO600nm = 0,8.


PHL818 culture ½ dilution (750µl culture + 750µl LB) and genomic DNA extraction on these 1,5ml with DNeasy Blood and Tissue Kit (QIAGEN). Note that 10µg of RNase A (Fermentas) had added and elution had been done with 200µl elution buffer, sample is finally stored at -20°C.








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