INSA-Lyon/16 August 2010
From 2010.igem.org
August 16
Second extraction of the L3 colonies
Liquid culture of I0500, 1F, 20P
Culture on plate of 18A/4O and 1F/24A (ligation of the August 14th)
Digestion with the OZYME kit :
- 24A, 10L and 14K : X+P
- 8C, 18A, 4O : E+S
- plasmide backbone Tet, plasmide backbone Cl : E+P
Ligation with the OZYME kit :
- 8C+14K in Tet backbone
- 10L+4O in Tet backbone
- 18A+24A in Cl backbone
Transformation of the ligation
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