INSA-Lyon/16 August 2010

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August 16


Second extraction of the L3 colonies

Liquid culture of I0500, 1F, 20P

Culture on plate of 18A/4O and 1F/24A (ligation of the August 14th)


Digestion with the OZYME kit :

  1. 24A, 10L and 14K : X+P
  2. 8C, 18A, 4O : E+S
  3. plasmide backbone Tet, plasmide backbone Cl : E+P

Ligation with the OZYME kit :

  1. 8C+14K in Tet backbone
  2. 10L+4O in Tet backbone
  3. 18A+24A in Cl backbone

Transformation of the ligation






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