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INSA-Lyon/16 August 2010
From 2010.igem.org
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| - | <p>Second extraction of the L3 colonies<br><br></p> | + | <p>Second extraction of the L3 colonies<br><br></p> |
| + | <p>Liquid culture of I0500, 1F, 20P </p> | ||
| + | <p>Culture on plate of 18A/4O and 1F/24A (ligation of the August 14th)</p><br> | ||
| + | <p>Digestion with the OZYME kit : <br> | ||
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| + | <ol id="list_extra"> | ||
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| + | <li>24A, 10L and 14K : X+P</li> | ||
| + | <li>8C, 18A, 4O : E+S </li> | ||
| + | <li>plasmide backbone Tet, plasmide backbone Cl : E+P</li> | ||
| + | <br> | ||
| + | </ol id="list_extra"></p> | ||
| + | <p>Ligation with the OZYME kit : <br> | ||
| + | <ol id="list_extra"> | ||
| + | <li>8C+14K in Tet backbone</li> | ||
| + | <li>10L+4O in Tet backbone</li> | ||
| + | <li>18A+24A in Cl backbone</li> | ||
| + | <br></ol id="list_extra"></p> | ||
| + | <p>Transformation of the ligation</p> | ||
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</html> | </html> | ||
Revision as of 10:03, 2 September 2010
August 16
Second extraction of the L3 colonies
Liquid culture of I0500, 1F, 20P
Culture on plate of 18A/4O and 1F/24A (ligation of the August 14th)
Digestion with the OZYME kit :
- 24A, 10L and 14K : X+P
- 8C, 18A, 4O : E+S
- plasmide backbone Tet, plasmide backbone Cl : E+P
Ligation with the OZYME kit :
- 8C+14K in Tet backbone
- 10L+4O in Tet backbone
- 18A+24A in Cl backbone
Transformation of the ligation
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