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INSA-Lyon/14 September 2010
From 2010.igem.org
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<li>Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation</li> | <li>Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation</li> | ||
<li>Digestion of 1F : ok</li> | <li>Digestion of 1F : ok</li> | ||
| + | <li>I0500,24A : no DNA</li> | ||
| + | <li>4O,10L : the weight of the DNA extraction is not correct, maybe a contamination</li> | ||
</ol><br></p> | </ol><br></p> | ||
Revision as of 08:29, 17 September 2010
September 14th
Ligation :
- 18A+L3 in Cl backbone
- 14+12/13 : in Cl backbone
- Curli+22B in Tet backbone
Digestion :
- pha A/pha B (clones 7 to 10) with E
- 8C, I0500 : with E+S
- 14K, 22B : with X+P
Transformation of the plasmid with phasin-intein
Agarose gel electrophoris for verification :
- Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation
- Digestion of 1F : ok
- I0500,24A : no DNA
- 4O,10L : the weight of the DNA extraction is not correct, maybe a contamination
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