INSA-Lyon/14 September 2010

From 2010.igem.org

(Difference between revisions)
m
 
Line 5: Line 5:
{{INSA-Lyon/menugauche}}
{{INSA-Lyon/menugauche}}
 +
{{INSA-Lyon/menunotebook}}
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en">
<html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" lang="en-US" xml:lang="en">
Line 29: Line 30:
});
});
</script>
</script>
-
 
-
<div class="colonnegauche">
 
-
<div id="menug">
 
-
    <ul>
 
-
<li><a href="/Team:INSA-Lyon/Project/Theory" class="cteal"> > Theory</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Strategy" class="slateb"> > Strategy</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Results" class="yellow"> > Results</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li>
 
-
 
-
<div id="ssmenug">
 
-
<li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li>
 
-
</div>
 
-
 
-
<li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modelling</a></li>
 
-
<li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li>
 
-
 
 
-
    </ul>
 
-
</div>
 
-
</div>
 

Latest revision as of 19:56, 25 October 2010





September 14th


Ligation :

  1. 18A+L3 in Cl backbone
  2. 14+12/13 : in Cl backbone
  3. Curli+22B in Tet backbone



Digestion :

  1. pha A/pha B (clones 7 to 10) with E
  2. 8C, I0500 : with E+S
  3. 14K, 22B : with X+P


Transformation of the plasmid with phasin-intein


Agarose gel electrophoris for verification :

  1. Clones 7 to 10 of the pha A/pha B ligation : none contain the ligation
  2. Digestion of 1F : ok
  3. I0500,24A : no DNA
  4. 4O,10L : the weight of the DNA extraction is not correct, maybe a contamination

Measure of fluorescence and DO on the PHL1273 culture

Start of 6 liquid cultures of PHL1273 waterbath at 28°C, 3 at 100 rpm, 3 at 140 rpm








June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31