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INSA-Lyon/14 October 2010
From 2010.igem.org
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<p>Transformation of the ligation of the 12/10 (with purified DNA)</p><br> | <p>Transformation of the ligation of the 12/10 (with purified DNA)</p><br> | ||
| - | <p>Liquid | + | <p>Liquid cultures of the ligations of the 12/10 (with unpurified DNA) : <br> |
<ol id="list_extra"> | <ol id="list_extra"> | ||
<li> phasin/phasin-intein : 4 clones</li> | <li> phasin/phasin-intein : 4 clones</li> | ||
<li>phasin-intein/2P: 7 clones</li> | <li>phasin-intein/2P: 7 clones</li> | ||
| - | <li>phaC/12/13 and Curli/phaC: too much clones, the ligation | + | <li>phaC/12/13 and Curli/phaC: too much clones, the ligation didn't work</li> |
<li>18A/L2/1N : no clones</li> | <li>18A/L2/1N : no clones</li> | ||
</ol><br></p> | </ol><br></p> | ||
Latest revision as of 21:33, 27 October 2010
October 14th
Transformation of the ligation of the 12/10 (with purified DNA)
Liquid cultures of the ligations of the 12/10 (with unpurified DNA) :
- phasin/phasin-intein : 4 clones
- phasin-intein/2P: 7 clones
- phaC/12/13 and Curli/phaC: too much clones, the ligation didn't work
- 18A/L2/1N : no clones
Digestion of the clones with X+P
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