http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_October_2010&feed=atom&action=history
INSA-Lyon/12 October 2010 - Revision history
2024-03-28T12:11:16Z
Revision history for this page on the wiki
MediaWiki 1.16.5
http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_October_2010&diff=197216&oldid=prev
MelanieGeffroy at 21:25, 27 October 2010
2010-10-27T21:25:55Z
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 21:25, 27 October 2010</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Liquid <del class="diffchange diffchange-inline">cultur </del>of 3 clones from the ligation 18A/OmpR234 of the 10/10</p><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Liquid <ins class="diffchange diffchange-inline">cultures </ins>of 3 clones from the ligation 18A/OmpR234 of the 10/10</p><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Digestion : <br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Digestion : <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol id="list_extra"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol id="list_extra"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phaA/phaB : with E</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phaA/phaB : with E</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol><br></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ol><br></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p><del class="diffchange diffchange-inline">Agarose </del>gel electrophoresis for verification and purification : <br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p><ins class="diffchange diffchange-inline">Agar </ins>gel electrophoresis for verification and purification : <br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol id="list_extra"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><ol id="list_extra"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>1N, L2/1N(E+P), curli and phaA/phaB : No DNA appear</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>1N, L2/1N(E+P), curli and phaA/phaB : No DNA appear</li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>18A + L2/1N in Amp backbone</li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>18A + L2/1N in Amp backbone</li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li>Curli + phaC in <del class="diffchange diffchange-inline">amp </del>backbone</li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li>Curli + phaC in <ins class="diffchange diffchange-inline">Amp </ins>backbone</li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phasin-intein/2P : in kan </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phasin-intein/2P : in kan </li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phaC + 12/13 </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li>phaC + 12/13 </li></div></td></tr>
</table>
MelanieGeffroy
http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_October_2010&diff=148642&oldid=prev
PYzou at 20:17, 25 October 2010
2010-10-25T20:17:21Z
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<td colspan='2' style="background-color: white; color:black;">Revision as of 20:17, 25 October 2010</td>
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<td colspan="2" class="diff-lineno">Line 1:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- {{INSA-Lyon_style}} --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- {{INSA-Lyon_style}} --></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/stylegroningen}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/stylegroningen}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/header}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/header}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/maincontent}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/maincontent}}</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/menugauche}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{INSA-Lyon/menugauche}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{INSA-Lyon/menunotebook}}</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> <ul></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Stage1" class="cteal"> > Stage 1</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Stage2" class="slateb"> > Stage 2</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Stage3" class="yellow"> > Stage 3</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><div id="ssmenug"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li></del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></div></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modelling</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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PYzou
http://2010.igem.org/wiki/index.php?title=INSA-Lyon/12_October_2010&diff=102408&oldid=prev
Magalbrun: New page: <!-- {{INSA-Lyon_style}} --> {{INSA-Lyon/stylegroningen}} {{INSA-Lyon/header}} {{INSA-Lyon/maincontent}} {{INSA-Lyon/menugauche}} <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" ...
2010-10-15T11:38:44Z
<p>New page: <!-- {{INSA-Lyon_style}} --> {{INSA-Lyon/stylegroningen}} {{INSA-Lyon/header}} {{INSA-Lyon/maincontent}} {{INSA-Lyon/menugauche}} <html xmlns="http://www.w3.org/1999/xhtml" dir="ltr" ...</p>
<p><b>New page</b></p><div><!-- {{INSA-Lyon_style}} --><br />
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{{INSA-Lyon/stylegroningen}}<br />
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ul#topnav li:hover {<br />
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<li><a href="/Team:INSA-Lyon/Project/Stage1" class="cteal"> > Stage 1</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Stage2" class="slateb"> > Stage 2</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Stage3" class="yellow"> > Stage 3</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Future_direction" class="coral"> > Future Direction</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Notebook" class="blue"> > Notebook</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Notebook/Calendar" class="cteal"> > Calendar</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Notebook/Protocols" class="brn"> > Protocols</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/Modeling" class="green"> > Modelling</a></li><br />
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<li><a href="/Team:INSA-Lyon/Project/References" class="orange"> > References</a></li><br />
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<h3>October 12th</h3><br />
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<p>Liquid cultur of 3 clones from the ligation 18A/OmpR234 of the 10/10</p><br><br />
<p>Digestion : <br><br />
<ol id="list_extra"><br />
<li>12/13 : with E+X</li><br />
<li>pha C with E+S</li><br />
<li>phasin-intein, 1N : with S+P</li><br />
<li>2P, L2/1N, phaC : with X+P</li><br />
<li>L2/1N, curli : with E+P</li><br />
<li>phaA/phaB : with E</li><br />
</ol><br></p><br />
<p>Agarose gel electrophoresis for verification and purification : <br><br />
<ol id="list_extra"><br />
<li>1N, L2/1N(E+P), curli and phaA/phaB : No DNA appear</li><br />
<li>12/13, pha C(X+P and E+S), phasin-intein, 2P, L2/1N (X+P) : OK purification</li><br />
</ol><br></p><br />
<p>Ligation overnight at 15°C , two series one with purified DNA and the other with DNA without purification: <br> <br />
<ol id="list_extra"><br />
<li>18A + L2/1N in Amp backbone</li><br />
<li>Curli + phaC in amp backbone</li><br />
<li>phasin-intein/2P : in kan </li><br />
<li>phaC + 12/13 </li><br />
<li>L2/1N + phaC</li><br />
<li>L2/1N + phasin-intein</li><br />
</ol><br></p><br />
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Magalbrun