INSA-Lyon/11 October 2010

From 2010.igem.org

(Difference between revisions)
m
 
Line 85: Line 85:
<li>phaA/phaB : with E</li>
<li>phaA/phaB : with E</li>
</ol><br></p>
</ol><br></p>
-
<p>Agarose gel electrophoresis fro verification and purification : <br>
+
<p>Agar gel electrophoresis fro verification and purification : <br>
<ol id="list_extra">
<ol id="list_extra">
<li>phasin and phasin-intein : OK, a lot of DNA, purification</li>
<li>phasin and phasin-intein : OK, a lot of DNA, purification</li>
-
<li>No DNA for all of the other digestion although there is DNA in the extraction</li>
+
<li>No DNA for all of the other digestions although there is DNA in the extraction</li>
</ol><br></p>
</ol><br></p>
-
<p>Cultur on plate of phaA/phaB, 12/13, 2P and L2/1N
+
<p>Cultures on plate of phaA/phaB, 12/13, 2P and L2/1N
<br><br><br><br><br><br><br>
<br><br><br><br><br><br><br>

Latest revision as of 21:24, 27 October 2010





October 11th


DNA extraction of phasin and phasin-intein

Digestion :

  1. Phasin-intein, phasin, 2P and L2/1N : with X+P
  2. Phaisn-intein, phasin, 1N and 18A : with S+P
  3. L2/1N : with E+P
  4. phaA/phaB : with E

Agar gel electrophoresis fro verification and purification :

  1. phasin and phasin-intein : OK, a lot of DNA, purification
  2. No DNA for all of the other digestions although there is DNA in the extraction

Cultures on plate of phaA/phaB, 12/13, 2P and L2/1N






June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31