INSA-Lyon/10 October 2010

From 2010.igem.org





October 10th


Result of the ligation of the 7/10 (1F/24A/14K and 18A/1N) : there was nothing. The ligations didn't work.


DNA extraction of 18A/OmpR234 and phasin/phasin-intein (ligation of the 7/10) : 2 clones of each one.

Digestion with E+P of the extraction


Transformation of the 5 ligations of yesterday and the part pha C.


Liquid cultures of 2 new clones for each ligation from the 7/10 (18A/OmpR234 and phasin/phasin-intein)


DNA extraction of 2P, L2/1N, pha A/pha B


Agar gel electrophoresis of the ligations and the PCR :

  1. 18A/OmpR234 : no insert
  2. phasin/phasin-intein : just one insert, phasin
  3. OmpR234 : a lot of DNA, we can see nothing
  4. pha A and pha B : a very thin band of DNA

Cultures on plate of phasin and phasin-intein








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