INSA-Lyon/10 October 2010
From 2010.igem.org
October 10th
Result of the ligation of the 7/10 (1F/24A/14K and 18A/1N) : there was nothing. The ligations didn't work.
DNA extraction of 18A/OmpR234 and phasin/phasin-intein (ligation of the 7/10) : 2 clones of each one.
Digestion with E+P of the extraction
Transformation of the 5 ligations of yesterday and the part pha C.
Liquid cultures of 2 new clones for each ligation from the 7/10 (18A/OmpR234 and phasin/phasin-intein)
DNA extraction of 2P, L2/1N, pha A/pha B
Agar gel electrophoresis of the ligations and the PCR :
- 18A/OmpR234 : no insert
- phasin/phasin-intein : just one insert, phasin
- OmpR234 : a lot of DNA, we can see nothing
- pha A and pha B : a very thin band of DNA
Cultures on plate of phasin and phasin-intein
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