Here

From 2010.igem.org

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We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. There are 39 reactions in total. A screenshot is provided below:
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We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:
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*LuxR does not form dimer;
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*AHL binds to LuxR and forms AHL-LuxR complex;
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*AHL-LuxR could form dimer, (AHL-LuxR)2;
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*Only (AHL-LuxR)2 could activate transcription from promoter lux pR;
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There are 12 species in total:
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*0:E_coli: E.coli cell in flask;
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*1:AHL: AHL added in the flask;
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*2:DNA: plasmid transformed into E.coli at the beginning;
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*3:AHL: AHL in E.coli because of diffusion;
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*4:RNA: mRNA of GFP;
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*5:RNA: mRNA of LuxR;
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*6:E0040_GFP: GFP protein;
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*7:C0062_LuxR: LuxR protein;
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*8:E0040_GFP_mature: mature GFP protein;
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*9:AHL;C0062_LuxR: complex of AHL and LuxR;
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*10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
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*11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;
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There are 39 reactions in total. A screenshot is provided below:
[[Image:USTCs Reaction1.PNG|700px|center]]
[[Image:USTCs Reaction1.PNG|700px|center]]

Revision as of 08:08, 25 October 2010

We present our modeling details of this AHL-induced LuxR-regulated lux pR-gfp system. We assume:

  • LuxR does not form dimer;
  • AHL binds to LuxR and forms AHL-LuxR complex;
  • AHL-LuxR could form dimer, (AHL-LuxR)2;
  • Only (AHL-LuxR)2 could activate transcription from promoter lux pR;

There are 12 species in total:

  • 0:E_coli: E.coli cell in flask;
  • 1:AHL: AHL added in the flask;
  • 2:DNA: plasmid transformed into E.coli at the beginning;
  • 3:AHL: AHL in E.coli because of diffusion;
  • 4:RNA: mRNA of GFP;
  • 5:RNA: mRNA of LuxR;
  • 6:E0040_GFP: GFP protein;
  • 7:C0062_LuxR: LuxR protein;
  • 8:E0040_GFP_mature: mature GFP protein;
  • 9:AHL;C0062_LuxR: complex of AHL and LuxR;
  • 10:(AHL)2;(C0062_LuxR)2: dimer of complex AHL-LuxR;
  • 11:(AHL)2;(C0062_LuxR)2;DNA: binding complex of AHL-LuxR dimer and lux pR on plasmid DNA;

There are 39 reactions in total. A screenshot is provided below:

USTCs Reaction1.PNG
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