HKU-Hong Kong/30 July 2010

From 2010.igem.org

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Contents

Work schedule 30 July 2010

  • confirmation of selected C1 C2 as transformed cell(from 26 July)
  • colony PCR/enzyme digestion of the 2 colonies (NcoI cut)

30jul.jpg

Results

Colony PCR

No bands can be observed. The two colonies are probably contaminations.

Enzyme digestion

Two bands were expected. Only one band of large size can be observed for both colonies, this is consistent with the conclusion drawn from the colony PCR.