13 July 2010:Con't BBa_k112808 isolation

Different combinations of enzymes were used for the isolation.

Result

Controls were run in parallel to the plasmid after various treatments. An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.