GrowingStreptomycesGR

From 2010.igem.org

Growing Streptomyces

Make plates with a thick layer of SFM medium, 100 mL medium per dish.

'''SFM medium''':

300 mL per 1 Liter bottle, to avoid boiling over in the autoclave.

- 6 gr agar
- 6 gr D-mannitol
- 6 gr soyaflower
- 300 mL demi-water

'''Plating''' 

- 300 uL streptomyces spores 10^5 diluted
- incubate for minimal 7 days at 30 degrees