Filamentous Cells
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'''Filamentous Cells''' | '''Filamentous Cells''' | ||
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Revision as of 14:18, 30 May 2010
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Filamentous Cells
SOS response is believe to be a universal bacteria phenomenon first studied in E.coli -LexA, recA |
In Bacillus subtillis (gram positive) dinR protein is homologous to lexA (Repressor of din-damage inducible genes).din genes include uvrA, uvrB, dinB, dinC dinR and recA. DNA damage inhibits cell division. |
Wild type Bacillus subtillis |
dinR KO |
dinR KO mutant over expressed the divergent (opposite direction) transcript for YneA, YneB and YnzC. These genes form the SOS regulon (recA independent SOS response) |
YneA suppressed in wt without SOS induction |
Expression of YneA from ITPG controlled promoter in wt leads to elongation. |
Disruption of YneA in SOS response leads to reduced elongation. Altering YneB and YnzC expression does not affect cell morphology. |
Double mutant (dinR/YneA) |
YneA protein required to suppress cell division. Not chromosome replication or segregation. |
FtsZ is important for bacterial cell division forming a ring structure at the division site by polymerising
assembling other proteins necessary for division at the site. |
FtsZ localises to the cell division cycle unless dinR is disrupted or YneA is being induced. |
YneA suppresses FtsZ ring formation- no proven direct interaction by two-hybrid. |
Filamentous cells less colony formation. |
YneA expression via the inactivation of dinR by Rec A is important. |
Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363. |