ExtractionCellWallsGR

From 2010.igem.org

Duisrupt your cells using a French press style cell disruptor, keep all collecting parts cool to reduce protease activity.


Repeat 6x, pressure: 13 kPSI


Spin off, 5 minutes at 4000 rpm.


Discard supernatant.


Cook pellet in 2% sds


Spin down, 4000 rpm.


Cook pellet in 2% SDS


Wash 10x


Dry freeze