BIOTEC Dresden/Notepad/9 August 2010

From 2010.igem.org

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Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids:
Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids:
  23e, 25a, 27e
  23e, 25a, 27e
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Gradient PCR using Phusion polymerase on plasmids:
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Gradient PCR using Fusion polymerase on plasmids:
  23e, 25a, 27e
  23e, 25a, 27e
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Assembly of the following parts were commenced with restriction digest and ligation:
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6e, 4a, 13e, 9b, 15e, 14f, 20f, 21f
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The plasmid backbone used was pSB1C3. Two ligations were performed - one based on the standard protocol and the other samples were left for overnight ligation. The ligated parts were transformed by electroporation method and plated.
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[[Category:BIOTEC Dresden/Notepad/August|August]]
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Latest revision as of 21:53, 27 October 2010

Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids:

23e, 25a, 27e

Gradient PCR using Fusion polymerase on plasmids:

23e, 25a, 27e

Assembly of the following parts were commenced with restriction digest and ligation:

6e, 4a, 13e, 9b, 15e, 14f, 20f, 21f 


The plasmid backbone used was pSB1C3. Two ligations were performed - one based on the standard protocol and the other samples were left for overnight ligation. The ligated parts were transformed by electroporation method and plated.




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