BIOTEC Dresden/Notepad/8 September 2010

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Fusion Protein

A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.



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