BIOTEC Dresden/Notepad/8 September 2010

From 2010.igem.org

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'''Parts Assembly'''
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Ligation of the digested samples from 07.09.2010 was done. A 1:1 and 1:3 ligation was done. The ligation mix was transformed by electroporation and plated and left overnight at 37 degrees.
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4a, 9b, 14f, 20f, 21f
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The concentration of all the plasmid backbones containing chloramphenicol was measured by nanodrop.
'''Fusion Protein'''
'''Fusion Protein'''
A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.  
A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.  
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[[Category:BIOTEC Dresden/Notepad/September|September]]
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{{Biotec_Dresden/month}}
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{{Biotec_Dresden/Bottom}}
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Latest revision as of 22:31, 27 October 2010

Parts Assembly

Ligation of the digested samples from 07.09.2010 was done. A 1:1 and 1:3 ligation was done. The ligation mix was transformed by electroporation and plated and left overnight at 37 degrees.

4a, 9b, 14f, 20f, 21f

The concentration of all the plasmid backbones containing chloramphenicol was measured by nanodrop.

Fusion Protein

A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.


July
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August
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September
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