BIOTEC Dresden/Notepad/4 October 2010

From 2010.igem.org

Parts Assembly

The sequencing results of the following parts were obtained. All the minipreps prepared were successful confirmed by the sequencing.

14f+6e, 21f+6e

Finally, the RBS was assembled with two reporter proteins (YFP and CFP).

Fusion Protein

Gradient PCR was done to plasmids pSB1C3 and pSB1T3 to determine the best temperature.

AHL Sensor

Digestion of part 51

Overnight cultures of parts 51 and 17 was prepared


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