http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&feed=atom&action=historyBIOTEC Dresden/Notepad/31 August 2010 - Revision history2024-03-29T10:18:23ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=198830&oldid=prevAdithya at 22:23, 27 October 20102010-10-27T22:23:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>AHL assay with part 18, for 3 hours, using AHL concentrations of 0.01-2000 nM, starting with O.D of 4.9.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>AHL assay with part 18, for 3 hours, using AHL concentrations of 0.01-2000 nM, starting with O.D of 4.9.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Category:BIOTEC Dresden/Notepad/August|August]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
</table>Adithyahttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=187353&oldid=prevSarah Mansour at 16:14, 27 October 20102010-10-27T16:14:34Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From these two positive clones overnight cultures were prepared. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From these two positive clones overnight cultures were prepared. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''AHL Sensor'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">AHL assay with part 18, for 3 hours, using AHL concentrations of 0.01-2000 nM, starting with O.D of 4.9.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td></tr>
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</table>Sarah Mansourhttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=132241&oldid=prevLucas.schirmer at 13:50, 24 October 20102010-10-24T13:50:11Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From these two positive clones overnight cultures were prepared. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>From these two positive clones overnight cultures were prepared. </div></td></tr>
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</table>Lucas.schirmerhttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=69607&oldid=prevMareike at 07:56, 13 September 20102010-09-13T07:56:21Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digest was heat inactivated for 20 minutes and then run on an agarose gel for confirming the success of the digest. The digests were fine and then gel purified after which concentration was measured.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digest was heat inactivated for 20 minutes and then run on an agarose gel for confirming the success of the digest. The digests were fine and then gel purified after which concentration was measured.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">'</del>'''Fusion Protein'''</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>'''Fusion Protein'''</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </div></td></tr>
</table>Mareikehttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=69606&oldid=prevMareike at 07:56, 13 September 20102010-09-13T07:56:09Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''Ligation of parts'''</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Restriction digest of following plasmid parts were repeated under the same conditions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Restriction digest of following plasmid parts were repeated under the same conditions.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digest was heat inactivated for 20 minutes and then run on an agarose gel for confirming the success of the digest. The digests were fine and then gel purified after which concentration was measured.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The digest was heat inactivated for 20 minutes and then run on an agarose gel for confirming the success of the digest. The digests were fine and then gel purified after which concentration was measured.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''''Fusion Protein'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">A colony PCR was performed to confirm the insertion of our fused insert into the plasmid pETMM43 or pETMMZZ. 8 clones from each plate were picked and PCR amplified. Analysis on an agarose gel revealed two positive clones for luxI-protein A as well as for the construct proteinA-luxI. </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">From these two positive clones overnight cultures were prepared. </ins></div></td></tr>
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</table>Mareikehttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=64639&oldid=prevCharansam21 at 13:55, 6 September 20102010-09-06T13:55:35Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Restriction digest of following plasmid parts were repeated under the same conditions.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> 4a, 6e, 9b, 14f, 20f, 21f</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">The digest was heat inactivated for 20 minutes and then run on an agarose gel for confirming the success of the digest. The digests were fine and then gel purified after which concentration was measured.</ins></div></td></tr>
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</table>Charansam21http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/31_August_2010&diff=50675&oldid=prevLucas.schirmer: New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> your txt here <hr> ...2010-08-14T19:47:59Z<p>New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> your txt here <hr> ...</p>
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