BIOTEC Dresden/Notepad/30 August 2010

From 2010.igem.org

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'''Fusion Protein'''
'''Fusion Protein'''
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The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out.  
+
The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour.
 +
The whole ligation reaction was used for electroporation.  

Revision as of 07:53, 13 September 2010

Fusion Protein

The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.




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