BIOTEC Dresden/Notepad/30 August 2010

From 2010.igem.org

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<p>Measurement of part 17e for 3 hours using AHL concentrations of (0.01-2000nM)
<p>Measurement of part 17e for 3 hours using AHL concentrations of (0.01-2000nM)
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[[Category:BIOTEC Dresden/Notepad/August|August]]
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Latest revision as of 22:21, 27 October 2010

Fusion Protein

The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.

AHL Sensor

AHL plate reader assay was done with part 24f. A pipetting scheme was used where different concentrations of the stock solution was used (from 0.015µM to 150µM) in LB.

Measurement of part 17e for 3 hours using AHL concentrations of (0.01-2000nM)


July
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August
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September
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October
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