BIOTEC Dresden/Notepad/30 August 2010

From 2010.igem.org

Fusion Protein

The ligation was set up again. Due to low plasmid concentrations only luxI-proteinA fusions were performed. Different molar ratios 1:1 and 1:3 were used. This time also control ligations using only the digested plasmid were carried out. Ligation reactions were incubated at room temperature for 1 hour. The whole ligation reaction was used for electroporation.

AHL Sensor

AHL plate reader assay was done with part 24f. A pipetting scheme was used where different concentrations of the stock solution was used (from 0.015µM to 150µM) in LB.

Measurement of part 17e for 3 hours using AHL concentrations of (0.01-2000nM)


July
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
August
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
September
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
October
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31

Share to Twitter Share to Facebook Share to Orkut Stumble It Email This More...