BIOTEC Dresden/Notepad/2 September 2010

From 2010.igem.org

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  4a, 14f, 20f, 21f
  4a, 14f, 20f, 21f
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'''Fusion Protein'''
'''Fusion Protein'''
To varify the correct insertion plasmids from positive colony PCR were sequenced.  
To varify the correct insertion plasmids from positive colony PCR were sequenced.  
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{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Revision as of 14:05, 24 October 2010

Five colonies for each of the following parts were picked and colony PCR was performed.

4a, 14f, 20f, 21f

Positive clones were identified and the corresponding cultures were left for overnight shaking.

Gradient PCR of primers for the parts and the chloramphenicol backbone was done and the right annealing temperature was determined.

4a, 14f, 20f, 21f

Fusion Protein

To varify the correct insertion plasmids from positive colony PCR were sequenced.


July
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