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BIOTEC Dresden/Notepad/27 September 2010
From 2010.igem.org
(Difference between revisions)
Charansam21 (Talk | contribs) (New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts Assembly''' ...) |
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The ligation mix was transformed by electroporation and plated and kept for overnight incubation. | The ligation mix was transformed by electroporation and plated and kept for overnight incubation. | ||
| - | + | '''Fusion Protein''' | |
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| + | DNA sequencing for the Fusion protein was sent | ||
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{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Revision as of 11:47, 23 October 2010
Parts Assembly
Restriction digest of following parts was done.
14f, 21f, 6e
The basic approach was to ligate the desired insert into the plasmid backbone containing the RBS insert. The digests were gel purified and concentration was measured. A 1:3 ligation was done for the following parts.
14f+6e, 21f+6e
The ligation mix was transformed by electroporation and plated and kept for overnight incubation.
Fusion Protein
DNA sequencing for the Fusion protein was sent
July |
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August |
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September |
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October |
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