http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&feed=atom&action=historyBIOTEC Dresden/Notepad/27 August 2010 - Revision history2024-03-28T15:28:15ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=198737&oldid=prevAdithya at 22:20, 27 October 20102010-10-27T22:20:07Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">[[Category:BIOTEC Dresden/Notepad/August|August]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
</table>Adithyahttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=132232&oldid=prevLucas.schirmer at 13:49, 24 October 20102010-10-24T13:49:41Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/month}}</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{Biotec_Dresden/Bottom}}</div></td></tr>
</table>Lucas.schirmerhttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=64626&oldid=prevCharansam21 at 13:52, 6 September 20102010-09-06T13:52:08Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Gel purification for the purified digests was done. Only bands corresponding to the backbone size were seen and the bands denoting the insert lengths were not seen. This may have been due to running the gel for too long or most of the DNA was lost during the purification step after the digest.</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Fusion Protein'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Fusion Protein'''</div></td></tr>
</table>Charansam21http://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=62422&oldid=prevMareike at 14:15, 2 September 20102010-09-02T14:15:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel. </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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</table>Mareikehttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=62421&oldid=prevMareike at 14:15, 2 September 20102010-09-02T14:15:15Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">your txt here</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">'''Fusion Protein'''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. </ins></div></td></tr>
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</table>Mareikehttp://2010.igem.org/wiki/index.php?title=BIOTEC_Dresden/Notepad/27_August_2010&diff=50671&oldid=prevLucas.schirmer: New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> your txt here <hr> ...2010-08-14T19:47:49Z<p>New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> your txt here <hr> ...</p>
<p><b>New page</b></p><div>{{Biotec_Dresden/Header}}<br />
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