BIOTEC Dresden/Notepad/27 August 2010

From 2010.igem.org

(Difference between revisions)
Line 13: Line 13:
All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul.  
All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul.  
 +
The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel.
<hr>
<hr>

Revision as of 14:15, 2 September 2010

Fusion Protein

All PCR purified parts were digested with the appropriate restriction enzymes for 1 hour at 37 °C. All double digested parts were again purified using the PCR purification kit. The concentration after purification was measured to be 40 - 150 ng/ul. The plasmids pETMM43 and pETMMZZ were double digested and purified from agarose gel.



July
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
August
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
September
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30
October
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31
Share to Twitter Share to Facebook Share to Orkut Stumble It Email This More...