BIOTEC Dresden/Notepad/22 September 2010

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AHL Sensor

Part 17 was measured in LB using a plate reader, with O.D 0.17.

Miniprep of the RFP plasmid and preparing their glycerol stocks.

Colonies were picked from parts 10, 11 and 17+51 for colony PCR. Fusion Protein PCR for the fusion parts followed by PCR purification. Restriction digest then purification of the digest


July
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August
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September
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October
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