BIOTEC Dresden/Notepad/1 September 2010

From 2010.igem.org

(Difference between revisions)
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The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.
The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.
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'''Fusion Protein'''
'''Fusion Protein'''
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.  
A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.  
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{{Biotec_Dresden/month}}
{{Biotec_Dresden/month}}
{{Biotec_Dresden/Bottom}}
{{Biotec_Dresden/Bottom}}

Revision as of 14:04, 24 October 2010

Ligation of following parts with 6e (RBS)

4a, 14f, 20f, 21f

The ligation mix was then transformed by electroporation and plated and kept for overnight incubation.

Fusion Protein

A Miniprep and glycerol stocks from the overnight cultures were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.


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