BIOTEC Dresden/Notepad/1 October 2010

From 2010.igem.org

(Difference between revisions)
(New page: '''Assembly of the amplifier-reporter part''' Part 45 (gel purified after restriction) was ligated into the linearized vector containing part 46 using the standard assembly method. The fi...)
Line 4: Line 4:
The final aim is to ligate the product with part one in the order 1-45-26  to produce the amplifier-reporter:
The final aim is to ligate the product with part one in the order 1-45-26  to produce the amplifier-reporter:
-
ptetR RBS LuxR Stop1 Stop2 luxRp       RBS RFP       RBS-luxI-Stop1-Stop2  
+
ptetR RBS LuxR Stop1 Stop2 luxRp         RBS RFP         RBS-luxI-Stop1-Stop2  
Ligation product showed the expected length in a restriction analysis  also sequencing results expected.
Ligation product showed the expected length in a restriction analysis  also sequencing results expected.

Revision as of 23:11, 4 October 2010

Assembly of the amplifier-reporter part

Part 45 (gel purified after restriction) was ligated into the linearized vector containing part 46 using the standard assembly method. The final aim is to ligate the product with part one in the order 1-45-26 to produce the amplifier-reporter:

ptetR RBS LuxR Stop1 Stop2 luxRp RBS RFP RBS-luxI-Stop1-Stop2

Ligation product showed the expected length in a restriction analysis also sequencing results expected.