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BIOTEC Dresden/Notepad/18 August 2010
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| - | + | The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours followed by heat inactivation. | |
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| + | Digestion was followed by a second purification with the pcr purification kit, concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the band seemed rather weak and was accompanied by a smear) | ||
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Revision as of 21:08, 18 August 2010
The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours followed by heat inactivation.
Digestion was followed by a second purification with the pcr purification kit, concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the band seemed rather weak and was accompanied by a smear)
July |
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August |
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September |
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October |
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