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BIOTEC Dresden/Notepad/18 August 2010
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| - | + | '''Parts Assembly''' | |
| + | The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed. | ||
| - | + | This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear) | |
| - | + | [[Category:BIOTEC Dresden/Notepad/August|August]] | |
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} | ||
Latest revision as of 21:59, 27 October 2010
Parts Assembly
The PCR products from the backbone amplification experiment were digested with DpnI restriction enzyme for 4 hours after which heat inactivation was performed.
This was followed by a second purification with the pcr purification kit, by concentration determination with nanodrop (today: in the range 60-80 not much lower than before digestion) and finally by running the samples on an agarose gel (although detectable, the bands seemed rather weak and were accompanied by a smear)
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
