BIOTEC Dresden/Notepad/16 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> your txt here <hr> ...) |
|||
Line 10: | Line 10: | ||
</html> | </html> | ||
- | + | '''AHL assay''' | |
+ | An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). | ||
+ | Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control. | ||
+ | Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm | ||
+ | |||
+ | |||
+ | However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB | ||
<hr> | <hr> |
Revision as of 06:39, 17 August 2010
AHL assay An AHL assay with part 24 (BBA_T9002) was carried in a 96 well plate with a step of 150 nM AHL concentration (range 150 - 1500 nM). Lb was used for blank, in addition pure bacterial culture (OD=6) was used as control.
Reading done at an emission wavelength of 535 nm and excitation wavelength 485 nm
However, a different amount of bacterial culture was used for wells of different AHL concentration, a necessary improvement for the next detection will be to introduce an equal nr of cells in each well and adjust the volume difference with LB
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |