BIOTEC Dresden/Notepad/14 October 2010

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(New page: {{Biotec_Dresden/Header}} <html> <head> <style type="text/css"> #bodyContent p, #bodyContent pre, #bodyContent table { margin:10px 30px; } </style> </head> </html> '''Parts Assembly''' ...)
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Restiction digest of the following parts was done for 1 hour 15 min at 37 degrees.
Restiction digest of the following parts was done for 1 hour 15 min at 37 degrees.
-
  53a, 53b, 53c, 54a, 54b, 54c, 55a, 55b, 55c
+
  1a, 4a, 6e+14f, 6e+21f, chloramphenicol backbone
 +
 
 +
Following parts were PCR purified and nanodropped.
 +
 
 +
1a, backbone
 +
 
 +
Following parts were gel purified and nanodropped.
 +
 
 +
4a, 6e+14f, 6e+21f
<hr>
<hr>

Revision as of 14:19, 21 October 2010

Parts Assembly

Restiction digest of the following parts was done for 1 hour 15 min at 37 degrees.

1a, 4a, 6e+14f, 6e+21f, chloramphenicol backbone

Following parts were PCR purified and nanodropped.

1a, backbone

Following parts were gel purified and nanodropped.

4a, 6e+14f, 6e+21f


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