BIOTEC Dresden/Notepad/12 August 2010

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The plasmid DNA concentration from ligation (A) were measured by Nanodrop. The concentration of the following plasmids were sufficient.

4a, 13e, 14f

The concentration of the following plasmids were very low.

15e, 20f, 21f

Fusion protein

PCR purified samples were tested on agarose gel.
Restriction digest of PCR purified samples 1-8 and plasmids -pETMM43(9) and pETMMZZ(10).
Ligation :Scheme
1+4+9
2+5+9
3+10
6+4+9
7+5+9
8+10
Incubation overnight / 1 hr.


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