BIOTEC Dresden/Notepad/12 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: {{Biotec_Dresden/Header}} '''Fusion protein''' PCR purified samples were tested on agarose gel. Restriction digest of PCR purified samples 1-8 and plasmids -pETMM43(9) and pETMMZZ(10). ...) |
|||
(6 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{Biotec_Dresden/Header}} | {{Biotec_Dresden/Header}} | ||
+ | <html> | ||
+ | <head> | ||
+ | <style type="text/css"> | ||
+ | #bodyContent p, #bodyContent pre, #bodyContent table { | ||
+ | margin:10px 30px; | ||
+ | } | ||
+ | </style> | ||
+ | </head> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | The plasmid DNA concentration from ligation (A) were measured by Nanodrop. The concentration of the following plasmids were sufficient. | ||
+ | |||
+ | 4a, 13e, 14f | ||
+ | |||
+ | The concentration of the following plasmids were very low. | ||
+ | |||
+ | 15e, 20f, 21f | ||
+ | |||
'''Fusion protein''' | '''Fusion protein''' | ||
PCR purified samples were tested on agarose gel. | PCR purified samples were tested on agarose gel. | ||
Line 11: | Line 30: | ||
8+10 | 8+10 | ||
Incubation overnight / 1 hr. | Incubation overnight / 1 hr. | ||
- | |||
<hr> | <hr> | ||
- | + | [[Category:BIOTEC Dresden/Notepad/August|August]] | |
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} |
Latest revision as of 21:55, 27 October 2010
The plasmid DNA concentration from ligation (A) were measured by Nanodrop. The concentration of the following plasmids were sufficient.
4a, 13e, 14f
The concentration of the following plasmids were very low.
15e, 20f, 21f
Fusion protein
PCR purified samples were tested on agarose gel. Restriction digest of PCR purified samples 1-8 and plasmids -pETMM43(9) and pETMMZZ(10). Ligation :Scheme 1+4+9 2+5+9 3+10 6+4+9 7+5+9 8+10 Incubation overnight / 1 hr.
July |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
August |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |
September |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | |
October |
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 31 |