2010.igem.org/Team:Harvard/fences/notebook

From 2010.igem.org

(Difference between revisions)
(June 14 2010)
 
Line 19: Line 19:
Plates incubated overnight, colonies observed in all plates except the control.
Plates incubated overnight, colonies observed in all plates except the control.
-
 
-
[[Image:Colonies 6-15-10.jpg|300px]]
 
-
[[Image:Colonies-2 6-15-10.jpg|300px]]
 
-
[[Image:Colonies-3 6-15-10.jpg|300px]]
 

Latest revision as of 16:29, 10 October 2010


Our lab notebook on OpenWetWare can be found [http://openwetware.org/wiki/IGEM:Harvard/2009/Notebook/Harvard_iGEM_2010 here].


June 14 2010

LacI Transformation

Performed bacterial transformation according to Silver:_Bacterial_Transformation, however we used 1μL in step 3, and used entire tube of TOP10 competent cells instead of only 10-15μL.

Transformed the following, each into its own tube of TOP10 E.Coli:

1) LacI with a rapid degredation tail attached, biobrick BBa_C0012, located on plate 1, well 2O, plasmid pSB1A2

2) LacI wildtype, biobrick BBa_I732100, located on plate 2, well 10E, plasmid pSB1A3

Streaked 100μL each of 1 and 2 onto separate LB + Amp dishes, along with a control dish of 100μL untransformed TOP10 E.Coli cells.

Plates incubated overnight, colonies observed in all plates except the control.