Team:BCCS-Bristol/Wetlab/0-10mM Assay

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Characterising the Biobrick - 0 - 10mM assay

Having established the biobrick was definitely switched on by 20mM of KNO3, we wanted to conduct a broader test of its behaviour across a range of different nitrate levels

An assay was set up between 0 – 10 mM of Potassium Nitrate at 2mM intervals. The same colony of MG1655 containing BBa_K381001 was used to inoculate six 5mL tubes of LB at varying nitrate levels as listed below. This was repeated twice to allow the results to be averaged

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