Team:Panama/27 July 2010

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iGEM Panama

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July 27

Miniprep Today we performed the miniprep of the E. coli transformed with the RBS and Promoter parts. This miniprep was made in triplicate.

Electrophoresis After the miniprep we made an electrophoresis in 1% agarose gel. Also, we measured the DNA concentration with a spectrophotometer:

[RBS]=30,4 ηg/μl

Digestion After the DNA concentration measurement, we performed the digestion of the RBS and Promoter parts.


Digestion mix for the Promoter:

NEBuffer2=5μl

BSA=0,5 μl

EcoRI=1 μl

SpeI=1 μl

DNA=20 μl

H2O=22,5μl

Digestion mix for the RBS:

NEBuffer2=5μl

BSA=0,5 μl

XbaI=1 μl

PstI=1 μl

DNA=20 μl

H2O=22,5μl

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May
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June
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July
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August
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September
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October
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