Team:HokkaidoU Japan/Notebook/October2

From 2010.igem.org

Revision as of 05:19, 2 October 2010 by Laurynas (Talk | contribs)

Notebook

Colony PCR

  • Performed Colonie PCR for 3 colonies which were incubated over night
  • Colony numbers were: 1, 2 and 3
  • Results showed no insertion
    • but when result came, had already done miniprep and prepared Sequencing Master Mix

Miniprep

  • Used Qiagen kit
  • Melted in 50 uL TE instead of H2O

Preparation for Sequencing

  • Mixed as shown in the table below


5x Sequencing Buffer 1.5uL 24.75 uL Ready Reaction Premix 1 uL 16.5 H2O 5 uL 80 uL total 7.5/sample

Reagent Amount Amount for 16.5
5x Sequencing Buffer 1.5uL 24.75 uL
Ready Reaction Premix 1 uL 16.5
H2O 5 uL 80 uL
Total 7.5/sample 121.25
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/October2"