Team:HokkaidoU Japan/Notebook/September13
From 2010.igem.org
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
Electrophoresed after gel extraction
- Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion
→Estimated concentration to be 54 ng/uL
- This time band location is good
- Accidentally excised a part of other band resulting small contamination
GFP(1-12O)ゲル抽精製済の濃度測定
- TSUDA I 2 uL, DNA 0.5 uL
→120 ng/uLと推定
- 878 + 220 bpが947 bpのマーカーのちょい上に来ている.OK.