Team:HokkaidoU Japan/Notebook/September7

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Notebook

Checked concentration of DNA used yestrday for ligation

  • 4 ng/uL RFP digested using either H buffer and M buffer
  • pSB1C3 band was very week

Ethanol percipication

In acordace to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol percipication was done.

コンピテントセルのPCR

先日のコロニーPCRで謎なバンドがすべてにみられたので，大腸菌ゲノム由来のものである可能性をみるため，コンピテントセルをPCRにかけたところ，バンドはみられなかった

  • すべてにみられたバンドはベクター由来か
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