Team:EPF Lausanne/Notebook/week9

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Contents

06-09-2010

Cloning

  • Western Blot started of C3-S-Immunotoxin to check if the Immunotoxin is expressed.

Drosophila

07-09-2010

Cloning

  • Western Blot from yesterday finished. Observed one band which did NOT correspond to the right size. Furthermore the negative control failed! Maybe it is another protein with a His-tag which is present on the C3 plasmid.

Drosophila

Other

*Gwen made us a chocolate cake!! Yeah! 

08-09-2010

Cloning

  • Western Blot from yesterday repeated. Colony PCR done to check if we are looking at the cells containing the correct plasmid.

Drosophila

Other

*Solange has a hangover! ;)